Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level.
Identifieur interne : 000506 ( Main/Exploration ); précédent : 000505; suivant : 000507Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level.
Auteurs : Martin Ern [République tchèque] ; Miroslav Berka [République tchèque] ; Hana Habánová [République tchèque]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Peptides, Protéines végétales.
- isolement et purification : Peptides, Protéines végétales.
- méthodes : Fractionnement chimique, Protéomique.
- Protéome, Solvants.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Peptides, Plant Proteins.
- chemical , isolation & purification : Peptides, Plant Proteins.
- methods : Chemical Fractionation, Proteomics.
- chemical : Proteome, Solvents.
Abstract
Peptide spectral libraries enable targeted identification and quantitation of low-abundance proteins in a complex plant proteome. Here we describe parallel protein and peptide fractionation techniques to improve plant proteome coverage and facilitate construction of spectral libraries.
DOI: 10.1007/978-1-4939-8814-3_6
PubMed: 30276734
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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